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蔡麗珠 教授













蔡麗珠 博士

實驗室: 生物分子實驗室 詳細資料

瑞典哥德堡大學 Göteborg University Ph.D.化學博士

辦公室:分子館 2 樓 (02)27712171 #2415 

研究室:分子館 4 樓 (02)27712171 #2436

學術榮譽: 95和97學年度本校本院優良研究獎




  1. 蛋白質/蛋白質與受質結構與功能分析
  2. 生物奈米材料與檢測
  3. 分子模擬


1. 蛋白質結構與功能的研究蛋白質的三級結構能讓人了解它們所扮演的角色和各式 各樣的功能。一個詳細的晶體結構,不僅說明了蛋白質結構與功能之密切關係,更有 助於基礎生物科學的發展,

也為酵素工程、新藥開發等生物科技,提供了不可或缺的 理論基礎。本實驗室主要是研究醣水解酶結構:包括家族六,十六與四十八的晶體結 構與受質。這些結構讓我們了解酵素水解機制與活性區氨基酸的關係。

2. 生物檢測元件(biosensors)的研究 生物分子的交互作用,如 DNA-DNA 或 DNA-enzymes 結合,抗體-抗原結合或解 離等催化反應會產生電荷性改變。

奈米場效應電晶體(NWFET)是一種高靈敏度且 可即時偵測矽線表面電荷改變所造成的電場訊息。 我們已經利用了 NWFET 的優勢 測得的最低濃度的靈敏度(0.1fM) DNA 雜交的能力(左下圖 )。

此外,也測得 大腸桿菌(右下圖),H1N1 病毒和氣體物質如乙醇和甲醇的,分子順式及反式兩種 構形也可檢測。



  1. Li-Chu Tsai, Hao-Ying Hsieh, Kun-Ying Lu, Sin-Yu Wang and Fwu-Long Mi*, “EGCG/gelatin-doxorubicin gold nanoparticles enhance therapeutic efficacy of doxorubicin for prostate cancer treatment”, Nanomedicne. (2016). 11(1), 9-30 (IF~6.2, R/C=12/123, Medicine, Research & Experimental)
  2. Li-Chu Tsai*, Imamaddin Amiraslanov, Hung-Ren Chen, Yun-Wen Chen, Hsiao-Lin Lee, Po-Huang Liang, and Yen-Chywan Liaw*, “Crystal structures of exoglucanase from Clostridium cellulovorans: cellotetraose binding and cleavage”, Acta Cryst. (2015). F71, 1264–1272 (IF~0.524, R/C=17/23, Crystallography) 3
  3. Yo-Chia Chen, Wan-Ting Chen, Jeng-Chen Liu, Li-Chu Tsai, Hsueh-Ling Cheng* “A highly active beta-glucanase from a new strain of rumen fungus Orpinomyces sp.Y102 exhibits cellobiohydrolase and cellotriohydrolase activities”, Bioresource Technology (2014), 170,513-521. (IF~=4.98, R/C=20/158, Biotehnology & Applied Microbiology)
  4. Chia-Jung Chu, Chia-Sen Yeh, Chun-Kai Liao, Li-Chu Tsai*, Chun-Ming Huang, Hung-Yi Lin*, Jing-Jong Shyue, Yit-Tsong Chen, and Chii-Dong Chen*, “Improving Nanowire Sensing Capability by Electrical Field Alignment of Surface Probing Molecules”, Nano Letter (2013), 13, 2564-2569 (IF~13.025, R/C=8/239, Materials science, Multidisciplinary)
  5. Yo-Chia Chen, Yu-Chuan Chiang, Fu-Yuan Hsu, Li-Chu Tsai*, Hsueh-Ling Cheng* “Structural modeling and further improvement in pH stability and activity of a highly-active xylanase from an uncultured rumen fungus”, Bioresource Technology (2012), 123,125-134. (IF~=4.98, R/C=20/158, Biotehnology & Applied Microbiology)
  6. Chih-Heng Lin, Chia-Jung Chu, Kang-Ning Teng, Yi-Jr Su, Chii-Dong Chen, Li-Chu Tsai, and Yuh-Shyong Yang “Recovery Based Nanowire Field-Effect Transistor Detection of Pathogenic Avian Influenza DNA”, Japanese Journal of Applied Physics 51 (2012) 02BL02-1-5, (IF~1.08, R/C=76/125, Physics, Applied) 
  7. Tsai, L. C.*, Hsiao, C. H., Liu, W. Y., Li-Ming Yin, L. M., and Shyur, L. F. “Structural basis for the inhibition of 1,3-1,4-β-D-glucanase by noncompetitive calcium ion and competitive Tris inhibitors”, Biochemical and Biophysical Research Communications (2011), 407, 953-958 (Biochemistry & Molecular biology, 146/283, SCI~2.55)
  8. Tsai, L. C.*, Cheng, I. C., Tu, M.C., Chen, C. D. and Lin, H. Y. “Formation of single-electron-transistors using self-assembly of nanoparticle chains”, Journal of Nanoparticle Research (2010), 12, 2859-2864. (IF~ 3.25, R/C= 24/66, Nanoscience & Nanotechnology
  9. Chen, J-H., Tsai, L. C.*, Huang, H.-C., and Shyur, L. F. “Structural and catalytic roles of amino acid residues located at substrate binding pocket in Fibrobacter succinogenes 1,3-1,4-β-D-glucanase”, Proteins: structure, function, and bioinformations (2010), 78, 4 2820-2830. (Biochemistry & Molecular biology, 111/283, 39.2%, SCI~3.09)
  10. Lin, Y.-S., Tsai, L. C., Lee, S. H., Yuan, H. S. and Shyur, L. F. “Structural and catalytic roles of residues located in β13 strand and the following β-turn loop in Fibrobacter succinogenes 1,3-1,4-β- D-glucanase”, Biochimica et Biophysica Acta (2009), 1790, 231-239 (Biochemistry&Molecular biology, 116/283, 41%, SCI~2.96)
  11. Tsai, L. C.*, Huang, H. C., Hsiao, C. H., Chiang, Y. N., Shyur, L. F., Lin, Y. S., and Lee, S. H. “Mutational and structural studies of the active site residues in truncated Fibrobacter succinogenes 1,3-1,4-β-D-glucanase”, Acta Crystallography (2008), D64, 1259-1266 (Crystallography, 4/25, 16%, SCI~2.94)
  12. Tsai, L. C.*, Chen, Y. N. and Shyur, L. F. “Structural Modeling of Glucanase−Substrate Complexes Suggests A Conserved Tyrosine is involved in Carbohydrate Recognition in Plant 1,3-1,4-β-D-Glucanases”, Journal of Computer Aided Molecular Design (2008) 22, 915-923 (Computer science, 3/94, 3.2%, SCI~3.62)
  13. Lin, M. C., Chu C. J., Tsai, L. C., Lin, H. Y., Wu, C. S., Wu, Y. P., Wu, Y. N., Shieh, D. B., Su, Y. W., and Chen C. D. “Control and detection of organosilane polarization on nanowire field-effect-transistors”, Nano Letter (2007), 7, 3656-3661 (Materials science, Multidisciplinary, 5/190, 2.6% SCI~9.63)
  14. Tsai, L. C.* “Rationale for Differential Carbohydrate Recognition by Barley and Fibrobacter succinogenes 1,3-1,4-β-D-Glucanases”, FBIT 2007 IEEE proceedings (2007), 311-316 ISBN: 978-0-7695-2999-8
  15. Lin, H. Y., Tsai, L. C., and Chen, C. D. “Assembly of Nanoparticle Patterns with Single-Particle Resolution Using DNA-Mediated Charge Trapping Technique: Method and Applications”, Advanced Functional Materials (2007), 17, 3182-3286. (Materials science, Multidisciplinary, 7/190, 3.7%, SCI~7.50) 
  16. Lin, H. Y., Tsai, L. C., Chi, P. Y., and Chen C. D “DNA as an Electron-Beam-Sensitive Reagent for Nanopatterning”, Advanced Materials (2006), 18, 1517-1520. (Materials science, Multidisciplinary, 6/176, 3.4%, SCI~7.90) 
  17. Tsai, L. C*., Shyur, L. F., Cheng, Y. S., and Lee, S. H. “Crystal Structure of Truncated Fibrobacter succinogenes 1,3-1,4-β-D-Glucanase in Complex with β-1,3-1,4-Cellotrios" Journal of Molecular Biology (2005), 354, 642-651. (Biochemistry & Molecular Biology 43/261, 16.5%, SCI~5.24)
  18. Lin, H. Y., Tsai, L. C., Chi, P. Y., and Chen C. D. “Positioning of extended individual DNA molecules between electrodes by non-uniform AC electric fields”, Nanotechnology (2005) 16, 2738-2742. (Nanoscience & nanotechnology, 5/27, 18.5%, SCI~2.99) 
  19. Tsai, L. C., Shyur, L. F., Lee, S. H., Lin, S. S., and Yuan, H. S. “Crystal structure of a natural jellyroll protein: circular-permutated 1,3-1,4-β-D-glucanase from Fibrobacter succinogenes” , J. Mol. Biol. (2003) 330, 607-620. (SCI~5.24)
  20. Li, C. L., Ho, L. I., Chang, Z. F., Tsai, L. C., Yang, W. Z. and Yuan, H. S. “DNA binding and cleavage by the periplasmic endonuclease Vvn from Vibrio Vulnificus: a novel structure with an old active site”, EMBO (2003) 22, 4014-4025. (SCI~10.46)
  21. Sui, M. J., Tsai, L. C., Hsia, K. C., Doudeva, L. G., Chak, K. F., and Yuan, H. S., “Metal ions and phosphate binding in the HNH motif: crystal structures of the nuclease domain of ColE7/Im7 in complex with a phosphate ion and Different Divalent Metal Ions” Protein Sci. (2002) 11,2947-2957. (SCI~3.79)
  22. Cheng, H. L., Tsai, L. C., Lin, Yuan, H. S., Lee, S. H., and Shyur, L. F. “Mutagenesis of Trp54 and Trp203 residues on Fibrobacter succinogenes 1,3-1,4-β-D-glucanase significantly affects the enzyme’s catalytic activities”, Biochem. (2002) 41, 8759-8766. (SCI~3.92)
  23. Yang, Z., Zhang, H., Hung, H. C., Kuo, C. C., Tsai, L. C., Yuan, H. S., Chou, W.Y., Chang, G. G., and Tong, L. “Structural studies of the pigeon cytosolic NADP(+)-dependent malic enzyme” Protein Sci. (2002) 11, 332-341. (SCI~3.79) 
  24. Tsai, L. C., Shyur, L. F., Lin, S. S., Yuan, H. S. “Crystallization and preliminary X-ray diffraction analysis of the truncated, Fibrobacter succinogenes 1,3-1,4-β-D-glucanase”, Acta Cryst. (2001) D57, 1303-1306.
  25. Chen, J. L., Tsai, L. C., Wen, T. N., Tang, J. B., Yuan, H. S. and Shyur, L F. “Directed mutagenesis of specific active site residues on Fibrobacter succinogenes 1,3-1,4- β -D-Glucanase significant affects catalysis and enzyme structural stability”, J. Biol. Chem., (2001) 276, 17895-17901.
  26. Kuo, C. C., Tsai, L. C., Chin, T. Y., Chang, G. G. and Chou, W. Y. “Lysine Residues 162 and 340 are involved in the catalysis and coenzyme binding of NADP+-dependent malic enzyme from pigeon”, Biochem. Biophys. Res. Comm. (2000) 270, 821-825.


  1. 黃曉娟,蔡麗珠* 
    Structural and functional study of the Eukaryotic Orpinomyces sp. Y102 OsCel6 catalytic domain C7 
    2013-12-7~10 在香港科技大學舉辦。   
  2. 蔡麗珠*,黃鼎,陳啟東 
    Detection of the interaction between enzyme and substrate by silicon nanowire field-effect-transistorsTaiwan-Mongolia Joint Symposium on Science Education
     2012-06-25-28 蒙古 烏蘭巴托 (口頭報告)
  3. 許富源,蔡麗珠*
     Structural and functional studies of the active-sites in four glycoside hydrolase b-jellyroll families
     第 22 屆國際結晶學會 
    The 22th Congress and General Assembly International Union of Crystallography 
    2011/8/22~30 西班牙馬德里 Venue: Madrid, Spain
  4. 蔡麗珠* ,黃曉娟,蕭敬樺,李韋儒,殷禮明 
    Structure and function of the Fibrobacter succinogenes 1,3-1,4-β-D-glucanase mutants F40I and W203F in complex with inhibitors 
    (The 10th Conference of the Asain Crystallographic Association) 
    2010/10/31~11/3 韓國釜山 Venue: Busan, Korea


以 DNA 作為電子束微影試劑以製作奈米圖案 
陳啟東, 林宏一, 紀佩吟, 蔡麗珠 
專利證書號碼: I 296045 
專利生效日期: 2008/4/21~2025/10/13
一種使用 DNA 為媒介之靜電捕捉技術作為 奈米顆粒組裝方法 
陳啟東, 林宏一,蔡麗珠 
專利證書號碼: I 348549 
專利生效日期: 2011/9/11~2027/11/2


# 計畫名稱 計畫內擔 任之工作 起迄年月 補助或委託機構 執行情形 經費總額 (萬) 
1 發展具有近紅外光熱效應與 
螢光影像之奈米金抗癌藥物 載體 
主持人 105/1~ 105/12 台北科技大學 台北醫學大學 執行中 50
2 矽奈米線元件應用於高靈敏 度生物分子的檢測 主持人 104/8~ 105/7 科技部 執行中 90
3 以矽奈米線元件檢測 F1-ATPase 與 ATP、 ADP

主持人 104/1~ 104/12 台北科技大學 台北醫學大學 完成 56.5
1 以奈米矽線場效應電晶體偵 
測酵素與醣類的相互結合與 催化作用 
主持人 103/1/1~ 103/12/31 台北科技大學 台北醫學大學 完成 45
1 纖維素水解酶結構與功能解 析

 NSC 101-2311-B-027 -001-
主持人 101/8~ 102/7 國科會 完成 72
1 非催化型多醣結合單元蛋白 之功能與結構的研究

 NSC 98-2311-B-027-001-MY3
主持人 98/8~ 101/7 國科會 完成 276.4